Goat Anti-NOX1 Antibody

$423.00

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SKU: EB10578
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
mitogenic oxidase 1| mitogenic oxidase (pyridine nucleotide-dependent superoxide-generating)| NOX-1| NADPH oxidase homolog-1| NADPH oxidase 1 variant NOH-1L| NADH/NADPH mitogenic oxidase subunit P65-MOX| MOX-1| NOH1| NOH-1| MOX1| GP91-2| NADPH oxidase 1|NOX1
Accession ID
NP_008983.2; NP_39249.1
Blocking Peptide
EBP10578
Immunogen
Peptide with sequence C-DKATDIVTGLKQK, from the internal region of the protein sequence according to NP_008983.2; NP_39249.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_008983.2; NP_39249.1).
Peptide Sequence
C-DKATDIVTGLKQK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
27035
Mouse Gene ID
237038
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 70-72kDa band observed in lysates of cell lines A549, CaCo-2, HeLa and U937, (calculated MW of 64.9kDa according to NP_008983.2;). This molecular weight is routinely observed by other sources and was successfully blocked by incubation with the immunising peptide. Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB