Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names STAT5A| STAT5| signal transducer and activator of transcription 5A| MGF | |
Usage Summary Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. | |
Accession ID NP_003143.2 | |
Blocking Peptide EBP11392 | |
Immunogen Peptide with sequence C-DSRLSPPAGLFTSAR, from the C Terminus of the protein sequence according to NP_003143.2. | |
Product Comments This antibody is not expected to cross-react with STAT5B. | |
Peptide Sequence C-DSRLSPPAGLFTSAR | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat | |
Reactive Species Human, Mouse | |
Human Gene ID 6776 | |
Mouse Gene ID 20850 | |
Rat Gene ID 24918 | |
Product Grade  | |
IHC Results Paraffin embedded Human Tonsil. Recommended concentration: 6-8µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 100kDa band observed in lysates of cell line NIH3T3 and in Mouse Lymph node lysates and approx. 90-100kDa in lysates of cell line K562 (calculated MW of 90.6kDa according to Human NP_003143.2 and 90.8kDa according to Mouse NP_001349609.1). Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IHC |
Goat Anti-STAT5A Antibody
$423.00
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Selected References [{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |