Goat Anti-CD28 Antibody

$423.00

Category: Primary Antibodies
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SKU: EB10740
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
T-cell-specific surface glycoprotein| CD28 antigen (Tp44)| CD28 antigen| Tp44| MGC138290| CD28 molecule|CD28
Accession ID
NP_006130.1
Blocking Peptide
EBP10740
Immunogen
Peptide with sequence SQQLQVYSKTGFNCD, from the internal region of the protein sequence according to NP_006130.1.
Product Comments
The immunizing peptide corresponds to a part of the extracellular domain.
Peptide Sequence
SQQLQVYSKTGFNCD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
940
Product Grade
IHC Results
In paraffin embedded Human Lymph Node shows membrane staining in the paracortex. Recommended concentration: 1-2µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Approx 48kDa band observed in Human Lymph Node and Tonsil lysates (calculated MW of 25.1kDa according to NP_006130.1). The observed molecular weight corresponds to the glycosylated form. Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC

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