Goat Anti-SDF4 (aa161-175) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

stromal cell-derived factor 4| calcium binding protein| SDF-4| OTTHUMP00000001754| 45 kDa calcium-binding protein| Cab45| stromal cell derived factor 4|SDF4

Immunofluorescence: Strong expression of the protein seen in the golgi and cytoplasm of U2OS cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration: 10ug/ml.

NP_057631.2; NP_057260.3

EBP10744

Peptide with sequence C-EYKVKFLASKGHSEK, from the internal region of the protein sequence according to NP_057631.2; NP_057260.3.

This antibody is expected to recognize both reported isoforms NP_057631.2; and NP_057260.3

C-EYKVKFLASKGHSEK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Human, Mouse, Rat, Dog, Cow

Human

51150

20318

155173

In paraffin embedded Human Kidney shows preferential staining in the brush borders of epithelial cells. Recommended concentration: 5-10µg/ml.

Antibody detection limit dilution 1:64000.

Approx 45kDa band observed in lysates of cell lines Jurkat, HeLa and U251 (calculated MW of 41.8kDa according to NP_057260.2). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.

Pep-ELISA, WB, IHC, IF, FC