Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names PR-domain zinc finger protein 14| PR domain-containing protein 14| PR domain zinc finger protein 14| PFM11| MGC59730| PR domain containing 14|PRDM14 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the nuclei of HeLa cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_078780.1 | |
Blocking Peptide EBP10761 | |
Immunogen Peptide with sequence YRDKHLKYTPCVDK, from the internal region of the protein sequence according to NP_078780.1. | |
Peptide Sequence YRDKHLKYTPCVDK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Human | |
Human Gene ID 63978 | |
Mouse Gene ID 383491 | |
Rat Gene ID 100365184 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:16000. | |
Western Blot Preliminary experiments gave an approx 40kDa band in Human Heart lysates after 0.1-1µg/ml antibody staining and this band was successfully blocked by incubation with the immunizing peptide. Preliminary experiments also gave an approxand an approx 48-50kDa band in Human Lung and Kidney lysates and in nuclear lysates of cell liines Jurkat, HEK293 and MCF7. Please note that currently we cannot find an explanation in the literature for these bands we observe given the calculated size of 64.1kDa according to Human NP_078780.1. | |
Application Type Pep-ELISA, IF, FC |
Goat Anti-PRDM14 (aa414-427) Antibody
$423.00
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