Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names plakophilin-2\| MGC177501\| ARVD9\| plakophilin 2\|PKP2
Accession ID NP_001005242.2; NP_004563.2
Blocking Peptide EBP10841
Immunogen Peptide with sequence C-RGTAQYSSQKSVEER, from the internal region of the protein sequence according to NP_001005242.2; NP_004563.2.
Product Comments This antibody is expected to recognize both reported isoforms (NP_001005242.2; NP_004563.2).
Peptide Sequence C-RGTAQYSSQKSVEER
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Human, Mouse, Rat, Dog, Cow
Reactive Species Human, Mouse
Human Gene ID 5318
Mouse Gene ID 67451
Rat Gene ID 287925
Product Grade
IHC Results Paraffin embedded Human Prostate. Recommended concentration: 3.75µg/ml.
ELISA Detection Limit Antibody detection limit dilution 1:16000.
Western Blot Approx 100kDa band observed in Mouse Heart lysates (calculated MW of 88.1kDa according to Mouse NP_080439.1 and 87.8kDa according to Rat NP_001093969.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Mouse, PMID: 37833253.
Application Type Pep-ELISA, WB, IHC

Selected References

[{"pmid": 37833253, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "MYH10 activation rescues contractile defects in arrhythmogenic cardiomyopathy (ACM).", "author": "Nieves García-Quintáns, Silvia Sacristán, Cristina Márquez-López, Cristina Sánchez-Ramos, Fernando Martinez-de-Benito, David Siniscalco, Andrés González-Guerra, Emilio Camafeita, Marta Roche-Molina, Mariya Lytvyn, David Morera, María I. Guillen, María A. Sanguino, David Sanz-Rosa, Daniel Martín-Pérez, Ricardo Garcia & Juan A. Berna", "journal": "Nat Commun. 2023 Oct 13;14(1):6461."}]

Goat Anti-Plakophilin 2 (aa125-139) Antibody

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