Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names central cannabinoid receptor\| cannabinoid receptor 1\| CNR\| CB1R\| CB1K5\| CB1A\| CB1\| CB-R\| CANN6\| cannabinoid receptor 1 (brain)\|CNR1
Accession ID NP_057167.2
Blocking Peptide EBP10961
Immunogen Peptide with sequence SNDIQYEDIKGDMAS-C, from the N Terminus of the protein sequence according to NP_057167.2.
Product Comments This antibody is expected to recognize reported isoform a (NP_057167.2) only. Reported variants represent identical protein: NP_001153731.1, NP_001153732.1, NP_057167.2, NP_001153730.1, NP_001153698.1
Peptide Sequence SNDIQYEDIKGDMAS-C
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Human, Mouse, Rat, Dog, Cow
Reactive Species Human, Mouse
Human Gene ID 1268
Mouse Gene ID 12801
Rat Gene ID 25248
Product Grade
ELISA Detection Limit Antibody detection limit dilution 1:64000.
Western Blot Approx 52kDa band observed in lysates of cell lines A549 and NIH3T3 (calculated MW of 52.9kDa according to Human NP_057167.2 and 52.8kDa according to Mouse NP_031752.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Human: 33049367 and http://doi.org/10.1089/can.2020.0107.
Application Type Pep-ELISA, WB

Selected References

[{"pmid": 33998898, "intro": "This antibody has been successfully used in WB on Human:", "title": "Differential Expression of CB1 Cannabinoid Receptor and Cannabinoid Receptor Interacting Protein 1a in Labor", "author": "Melissa L. Kozakiewicz, Jie Zhang, Sandra Leone-Kabler, Liliya M. Yamaleyeva, Anna G. McDonald, Brian C. Brost and Allyn C. Howlett", "journal": "Cannabis and Cannabinoid Research (April 2021) http://doi.org/10.1089/can.2020.0107"}, {"pmid": 33049367, "intro": "This antibody has been successfully used in WB on Human:", "title": "Cannabinoid receptor subtype influence on neuritogenesis in human SH-SY5Y cells", "author": "Erica L Lyons, Sandra Leone-Kabler, Alexander L Kovach, Brian F Thomas, Allyn C Howlett", "journal": "Mol Cell Neurosci. 2020 Oct 10;109:103566."}]

Goat Anti-CB1 (isoform a) Antibody

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