Goat Anti-Histamine Receptor H2 (mouse) Antibody

$255.00

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SKU: EB11019
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
Hrh2| histamine receptor H2| H2R| HH2R| gastric receptor 1| gastric receptor I| histamine H2 receptor| gastric receptor 1| gastric receptor I| H2R| HH2R| histamine H2 receptor| histamine receptor H2|Hrh2
Accession ID
NP_001010973.1; NP_032312.2
Blocking Peptide
EBP11019
Immunogen
Peptide with sequence C-NRNSHKTSLRSNASQ, from the internal region of the protein sequence according to NP_001010973.1; NP_032312.2.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_001124527.1; NP_071640.1).
Peptide Sequence
C-NRNSHKTSLRSNASQ
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse, Rat
Mouse Gene ID
15466
Rat Gene ID
25461
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:64000.
Western Blot
Preliminary experiments gave an approx 27kDa band in Mouse Brain lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 44.8kDa according to NP_001010973.1. The 27kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA