Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names TBK1| TANK-binding kinase 1| T2K| serine/threonine-protein kinase TBK1| NF-kB-activating kinase| NF-kappa-B-activating kinase| NAK| FLJ11330 | |
Usage Summary Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 and in the cytoplasm and ER of HeLa cells. Recommended concentration: 10µg/ml.
Flow Cytometry: Flow cytometric analysis of HeLa cells. Recommended concentration: 10ug/ml. | |
Accession ID NP_037386.1 | |
Blocking Peptide EBP11055 | |
Immunogen Peptide with sequence C-TIETSLQDIDSRLS, from the internal region of the protein sequence according to NP_037386.1. | |
Peptide Sequence C-TIETSLQDIDSRLS | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human | |
Reactive Species Human | |
Human Gene ID 29110 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 80kDa band observed in lysates of cell line LNCaP and A431 (calculated MW of 83.6kDa according to NP_037386.1). Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature. | |
Application Type Pep-ELISA, WB, IF, FC |
Goat Anti-TBK1 (aa514-527) Antibody
$423.00
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Selected References [{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |