Goat Anti-Adam12 (mouse) Antibody

$255.00

Category: Primary Antibodies
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SKU: EB11060
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
a disintegrin and metallopeptidase domain 12 (meltrin alpha)| a disintegrin and metalloprotease domain 12| a disintegrin and metalloproteinase domain 12 (meltrin alpha)| ADAM 12| disintegrin and metalloproteinase domain-containing protein 12| KIAA4001| meltrin alpha| meltrin-alpha| mKIAA4001| Mltna| OTTMUSP00000023508|Adam12
Accession ID
NP_031426.2
Blocking Peptide
EBP11060
Immunogen
Peptide with sequence KKTTMEKLRCVHP, from the internal region of the protein sequence according to NP_031426.2.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_003465.3; NP_067673.2).
Peptide Sequence
KKTTMEKLRCVHP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Mouse, Rat, Pig
Human Gene ID
8038
Mouse Gene ID
11489
Rat Gene ID
679837
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:4000.
Western Blot
Preliminary experiments gave an approx 37kDa band in Mouse and Rat Skeletal Muscle lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 98.5kDa according to NP_031426.2. The 37kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA

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