Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names PRODH\| tumor protein p53 inducible protein 6\| TP53I6\| SCZD4\| proline oxidase, mitochondrial\| proline oxidase 2\| proline dehydrogenase, mitochondrial\| proline dehydrogenase (oxidase) 1\| PRODH2\| PRODH1\| POX\| PIG6\| p53-induced gene 6 protein\| OTTHUMP00000196497\| OTTHUMP00000196496\| MGC148079\| MGC148078\| HSPOX2\| FLJ33744
Accession ID NP_057419.4; NP_001182155.1
Blocking Peptide EBP11136
Immunogen Peptide with sequence C-EDQESIQPLLRHYR, from the internal region of the protein sequence according to NP_057419.4; NP_001182155.1.
Product Comments This antibody is expected to recognize both reported isoforms (NP_057419.4; NP_001182155.1).
Peptide Sequence C-EDQESIQPLLRHYR
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Human
Reactive Species Human
Human Gene ID 5625
Product Grade
ELISA Detection Limit Antibody detection limit dilution 1:16000.
Western Blot Approx75+55kDa bands observed in Human Brain (Cerebellum and Substantia nigra) lysates (calculated MW of 68.0kDakDa according to NP_057419.4 and of 56.2kDa according to NP_001182155.1). Recommended concentration: 0.3-1µg/ml.
Application Type Pep-ELISA, WB

Selected References

[{"pmid": 34085157, "intro": "This antibody has been successfully used in WB on Human:", "title": "Proline oxidase silencing inhibits p53-dependent apoptosis in MCF-7 breast cancer cells.", "author": "Ilona Oscilowska, Thi Y. L. Huynh, Weronika Baszanowska, Izabela Prokop, Arkadiusz Surazynski, Mauro Galli, Piotr Zabielski & Jerzy Palka", "journal": "Amino Acids. 2021 Dec;53(12):1943-1956."}, {"pmid": 28942439, "intro": "This antibody has been successfully used in Western blot on Human:", "title": "Functional Consequences of Intracellular Proline Levels Manipulation Affecting PRODH/POX-Dependent Pro-Apoptotic Pathways in a Novel in Vitro Cell Culture Model.", "author": "Zareba I, Surazynski A, Chrusciel M, Miltyk W, Doroszko M, Rahman N, Palka J.", "journal": "Cell Physiol Biochem. 2017 Sep 22;43(2):670-684."}]

Goat Anti-PRODH (aa112-134) Antibody

Related products