Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names Cyp1a2| P450-3| cytochrome P450-P3| cytochrome P450-P2| cytochrome P450, family 1, subfamily A, polypeptide 2| cytochrome P450, 1a2, aromatic compound inducible| cytochrome P450 family 1 subfamily a polypeptide 1| cytochrome P450 1A2| CYPIA2| Cyp1a1| CP12| aromatic compound inducible | |
Accession ID NP_034123.1 | |
Blocking Peptide EBP11171 | |
Immunogen Peptide with sequence C-HSENYKDNGGLIPEEK, from the internal region of the protein sequence according to NP_034123.1. | |
Product Comments This antibody is not expected to cross-react to the similar P450 1A1. | |
Peptide Sequence C-HSENYKDNGGLIPEEK | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Mouse, Rat | |
Reactive Species Human, Mouse, Rat | |
Mouse Gene ID 13077 | |
Rat Gene ID 24297 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:32000. | |
Western Blot Approx 60kDa band observed in Mouse fetal Liver lysates and approx. 55kDa in Human Liver and Lung lysates (calculated MW of 58.4kDa according to Human NP_000752.2 and 58.2kDa according to Mouse NP_034123.1). Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Rat Liver lysates, PMID: 35094979. | |
Application Type Pep-ELISA, WB |
Goat Anti-Cytochrome P450 1A2 (mouse) Antibody
$423.00
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Selected References [{"pmid": 35094979, "intro": "This antibody has been successfully used in Western blot on Rat:", "title": "Proteomic and Bioinformatics Analysis of Membrane Lipid Domains after Brij 98 Solubilization of Uninduced and Phenobarbital-Induced Rat Liver Microsomes: Defining the Membrane Localization of the P450 Enzyme System", "author": "James R Reed, Jessie J Guidry, Wayne L Backes", "journal": "Drug Metab Dispos. 2022 Apr;50(4):374-385."}] |