Goat Anti-ULK3 (aa445-458) Antibody

$423.00

SKU Unit Size Price

Select a unit size:


SKU: EB11184
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
DKFZp434C131| FLJ90566| serine/threonine-protein kinase ULK3| unc-51-like kinase 3 (C. elegans)|ULK3
Usage Summary
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Accession ID
NP_001092906.1
Blocking Peptide
EBP11184
Immunogen
Peptide with sequence C-KMRESRWEADTLDK, from the C Terminus of the protein sequence according to NP_001092906.1.
Peptide Sequence
C-KMRESRWEADTLDK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Pig, Dog
Reactive Species
Human
Human Gene ID
25989
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 50kDa band observed in Human Brain (Cerebellum, Cerebral Cortex, Hippocampus) lysates (calculated MW of 53.4kDa according to NP_001092906.1). Recommended concentration: 1-3µg/ml. An additional band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide.
Application Type
Pep-ELISA, WB
Selected References
[{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]