Goat Anti-Zfp652 (mouse) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

RP23-151M22.1| 9530033F24Rik| MGC67134| OTTMUSP00000001678| OTTMUSP00000001680| OTTMUSP00000037466| zinc finger protein 652| ZNF652|Zfp652

NP_963903.2

EBP11189

Peptide with sequence C-RHLAEKNSAAPAQHH, from the C Terminus of the protein sequence according to NP_963903.2.

Reported variants represent identical protein: NP_055712.1, NP_001138837.1

C-RHLAEKNSAAPAQHH

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Mouse, Rat

268469

497984

Antibody detection limit dilution 1:16000.

Preliminary experiments gave bands at approx 45kDa and 18kDa in Mouse Heart lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the bands we observe given the calculated size of 69.4kDa according to NP_963903.2. Both detected bands were successfully blocked by incubation with the immunizing peptide (and BLAST results with the immunizing peptide sequence did not identify any other proteins to explain the additional bands). We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Could the bands represent cleaved products?

Pep-ELISA