Goat Anti-TRIM6 (aa118-131) Antibody

$255.00

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SKU: EB11372
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
OTTHUMP00000069818| OTTHUMP00000069819| OTTHUMP00000069820| OTTHUMP00000197092| OTTHUMP00000220648| OTTHUMP00000220649| OTTHUMP00000220650| RING finger protein 89| RNF89| tripartite motif containing 6| tripartite motif-containing 6| tripartite motif-containing protein 6|TRIM6
Accession ID
NP_001003818.1; NP_477514.1
Blocking Peptide
EBP11372
Immunogen
Peptide with sequence KQLKAVLCADHGEK, from the internal region of the protein sequence according to NP_001003818.1; NP_477514.1.
Product Comments
This antibody is expected to recognize reported isoforms 1 and 2 (NP_001003818.1; NP_477514.1). Reported variants represent identical protein: NP_001185573.1, NP_001185574.1
Peptide Sequence
KQLKAVLCADHGEK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Human Gene ID
117854, 445372
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:8000.
Western Blot
Preliminary experiments gave an approx 70-75kDa band in Human Kidney and Liver lysates after 0.2µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 59.4kDa according to NP_001003818.1. The 70-75kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA