Goat Anti-ZAP70 Antibody

$423.00

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SKU: EB11394
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
70 kDa zeta-associated protein| FLJ17670| FLJ17679| SRK| STD| syk-related tyrosine kinase| tyrosine-protein kinase ZAP-70| TZK| ZAP-70| zeta-chain (TCR) associated protein kinase 70kDa| zeta-chain associated protein kinase, 70kD|ZAP70
Usage Summary
Immunoprecipitation:This antibody was deemed fit for IP under native conditions (observations from customer).

Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

Accession ID
NP_001070.2
Blocking Peptide
EBP11394
Immunogen
Peptide with sequence C-SDGYTPEPARITSPD, from the internal region of the protein sequence according to NP_001070.2.
Product Comments
This antibody is expected to recognize isoform 1 (NP_001070.2) only.
Peptide Sequence
C-SDGYTPEPARITSPD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
7535
Mouse Gene ID
22637
Rat Gene ID
301348
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:32000.
Western Blot
Approx 70kDa band observed in lysates of cell lines Jurkat and Molt4 (calculated MW of 69.9kDa according to NP_001070.2). Recommended concentration: 0.3-1µg/ml.
Application Type
Pep-ELISA, WB, IP
Selected References
[{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]