Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names RRAD| REM3| Ras-related associated with diabetes| ras associated with diabetes| RAS (RAD and GEM) like GTP binding 3| RAD1| RAD| OTTHUMP00000174805| GTP-binding protein RAD | |
Accession ID NP_004156.1 | |
Blocking Peptide EBP11418 | |
Immunogen Peptide with sequence C-HRRSMPVDERDLQ, from the internal region of the protein sequence according to NP_004156.1. | |
Product Comments Reported variants represent identical protein: NP_004156.1, NP_001122322.1 | |
Peptide Sequence C-HRRSMPVDERDLQ | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Rat, Dog, Pig, Cow | |
Reactive Species Mouse | |
Human Gene ID 6236 | |
Mouse Gene ID 56437 | |
Rat Gene ID 83521 | |
Product Grade  | |
ELISA Detection Limit Antibody detection limit dilution 1:64000. | |
Western Blot Approx 37kDa band observed in Mouse Heart lysates and no 37kDa in the KO lysates (calculated MW of 33.2kDa according to mouse NP_062636.2 and human NP_004156.1). There is some non-specific background, we call for caution when used for other assays than Western blot. In transfected HEK293 transiently expressing Mouse Rrad (HA tagged), a band of approx. 45kDa is observed. No bands are observed in transfected HEK293 transiently expressing related Mouse GTPases. Recommended concentration: 0.5-2µg/ml. | |
Application Type Pep-ELISA, WB |
Goat Anti-RRAD (aa36-48) Antibody
$423.00
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Selected References [{"pmid": 28732776, "intro": "This antibody has been successfully used in Western blot on Mouse:", "title": "Rad GTPase is essential for the regulation of bone density and bone marrow adipose tissue in mice", "author": "Catherine N. Withers, Drew M. Brown, Innocent Byiringiro , Matthew R. Allen , Keith W. Condon, Jonathan Satin, Douglas A. Andres", "journal": "Bone. 2017 Oct; 103: 270-280."}] |