Goat Anti-SERCA2 / ATP2A2 Antibody

$464.00

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SKU: EB11450
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ATP2A2| SR Ca(2+)-ATPase 2| SERCA2| sarcoplasmic/endoplasmic reticulum calcium ATPase 2| MGC45367| FLJ38063| FLJ20293| endoplasmic reticulum class 1/2 Ca(2+) ATPase| DKFZp686P0211| DD| DAR| cardiac Ca2+ ATPase| calcium-transporting ATPase sarcoplasmic reticulum type, slow twitch skeletal muscle isoform| calcium pump 2| ATPase, Ca++ transporting, cardiac muscle, slow twitch 2| ATPase, Ca++ dependent, slow-twitch, cardiac muscle-2| ATP2B
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the endoplasmic reticulum of HeLa cells. Recommended concentration: 10µg/ml.
Accession ID
NP_001672.1; NP_733765.1
Blocking Peptide
EBP11450
Immunogen
Peptide with sequence DELNPSAQRDACLN, from the internal region of the protein sequence according to NP_001672.1; NP_733765.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_001672.1; NP_733765.1).
Peptide Sequence
DELNPSAQRDACLN
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig
Reactive Species
Human, Mouse, Rat
Human Gene ID
488
Mouse Gene ID
11938
Rat Gene ID
29693
Product Grade
IHC Results
Paraffin embedded Human Heart. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx. 110kDa band observed in Human, Mouse and Rat Heart lysates and in lysates of cell line A549, and approx. 100kDa in Mouse Smooth Muscle lysates (calculated MW of 110kDa according to Human NP_001672.1, Mouse NP_033852.1 and Rat NP_001103609.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, IHC