Goat Anti-Estrogen receptor beta (aa417-428) Antibody

$423.00

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SKU: EB11581
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
OTTHUMP00000180647|nuclear receptor subfamily 3 group A member 2|NR3A2|estrogen receptor beta 4|estrogen receptor beta|estrogen receptor 2 (ER beta)|estrogen nuclear receptor beta variant b|estrogen nuclear receptor beta variant a|ESTRB|ESR-BETA|ESRB|ESR2|ER-BETA|Erb
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the nucleus of HeLa and MCF7 cells. Recommended concentration: 10µg/ml.
Accession ID
NP_001428.1; NP_001035365.1; NP_001201831.1; NP_001201832.1
Blocking Peptide
EBP11581
Immunogen
Peptide with sequence C-TQDADSSRKLAH, from the internal region of the protein sequence according to NP_001428.1; NP_001035365.1; NP_001201831.1; NP_001201832.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_001428.1; NP_001035365.1; NP_001201831.1; NP_001201832.1). Reported variants represent identical protein: NP_001035365.1, NP_001035366.1
Peptide Sequence
C-TQDADSSRKLAH
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
2100
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 50kDa band observed in Human Placenta lysates (calculated MW of 53.0kDa according to NP_001035366.1). Recommended concentration: 1-3µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IF