Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names protein kinase C, beta 1 polypeptide|protein kinase C, beta|protein kinase C beta type|PRKCB2|PRKCB1|PRKCB|PKC-beta|PKC-B|PKCB|OTTHUMP00000162378|OTTHUMP00000162377|MGC41878 | |
Usage Summary Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371. | |
Accession ID NP_997700.1 | |
Blocking Peptide EBP11585 | |
Immunogen Peptide with sequence C-DKEFTRQPVELT, from the internal region (near C terminus) of the protein sequence according to NP_997700.1. | |
Product Comments This antibody is expected to recognize isoform 1 (NP_997700.1) only. | |
Peptide Sequence C-DKEFTRQPVELT | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Rat, Dog | |
Reactive Species Human, Rat | |
Human Gene ID 5579 | |
Product Grade  | |
IHC Results In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 80kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9kDa according to NP_997700.1). Recommended concentration: 0.1-0.3µg/ml. | |
Application Type Pep-ELISA, WB, IHC |
Goat Anti-PRKCB Antibody
$423.00
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Selected References [{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}] |