Goat Anti-CDC48 / YDL126C Antibody

$423.00

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SKU: EB11800
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
TERA| p97| valosin containing protein| transitional endoplasmic reticulum ATPase|YDL126C|VCP|transitional endoplasmic reticulum ATPase|CDC48p|CDC48
Usage Summary
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Accession ID
NP_010157.1
Blocking Peptide
EBP11800
Immunogen
Peptide with sequence C-HTKNMKLADDVDLE, from the internal region of the protein sequence according to NP_010157.1.
Product Comments
This antibody may cross react in many other species.
Peptide Sequence
C-HTKNMKLADDVDLE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Saccharomyces cerevisiae S288c; Human, Mouse, Rat, Dog, Cow
Reactive Species
Human
Human Gene ID
7415
Mouse Gene ID
269523
Rat Gene ID
116643
Product Grade
IHC Results
Paraffin embedded Human Placenta. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 100kDa band observed in lysates of cell line HeLa (calculated MW of 92.0kDa according to NP_010157.1). Recommended concentration: 0.01-0.03µg/ml.
Application Type
Pep-ELISA, WB, IHC
Selected References
[{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]