Goat Anti-DNAJC5G (human) Antibody

$423.00

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SKU: EB11850
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
CSP-gamma|cysteine string protein-gamma|DNAJ (Hsp40) homolog, subfamily C, member 5 gamma|DNAJ homolog subfamily C member 5G|DNAJC5G|FLJ40417|gamma cysteine string protein|gamma-CSP|gamma-cysteine string protein
Accession ID
NP_775921.1
Blocking Peptide
EBP11850
Immunogen
Peptide with sequence C-DSGRKYQQNVQSQP, from the internal region (near C Terminus) of the protein sequence according to NP_775921.1.
Peptide Sequence
C-DSGRKYQQNVQSQP
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human
Human Gene ID
285126
Product Grade
IHC Results
In paraffin embedded Human Testis shows nuclear staining primarily in spermatogonia and in cellular membrane. Recommended concentration, 5-10µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Preliminary experiments gave an approx 28kDa band in Human Testis lysates after 0.05µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 21.4kDa according to provisional NP_775921.1. The 28kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Application Type
Pep-ELISA, IHC