Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names plasma membrane adaptor HA2/AP2 adaptin alpha A subunit\| clathrin-associated/assembly/adaptor protein, large, alpha 1\| clathrin assembly protein complex 2 alpha-A large chain\| alpha1-adaptin\| alpha-adaptin A\| adaptor protein complex AP-2 subunit alpha-1\| adaptin, alpha A\| adapter-related protein complex 2 alpha-1 subunit\| AP-2 complex subunit alpha-1\| 100 kDa coated vesicle protein A\| CLAPA1\| AP2-ALPHA\| ADTAA\| adaptor-related protein complex 2, alpha 1 subunit\|AP2A1
Accession ID NP_055018.2
Blocking Peptide EBP11875
Immunogen Peptide with sequence C-ELEPPAPESPMALLADPAPAAD, from the internal region of the protein sequence according to NP_055018.2.
Product Comments This antibody is expected to recognize reported isoform 1 (NP_055018.2) only.
Peptide Sequence C-ELEPPAPESPMALLADPAPAAD
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Human, Mouse, Rat, Pig, Cow
Reactive Species Human
Human Gene ID 160
Mouse Gene ID 11771
Rat Gene ID 308578
Product Grade
IHC Results Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5-7µg/ml.
ELISA Detection Limit Antibody detection limit dilution 1:128000.
Western Blot Approx 110kDa band observed in Human Brain (Frontal cortex) lysates (calculated MW of 108kDa according to NP_055018.2). Recommended concentration: 0.03-0.1µg/ml. Primary incubation was 1 hour.
Application Type Pep-ELISA, WB, IHC

Selected References

[{"pmid": 35314489, "intro": "This antibody (previous batch) has been successfully used in IF on Human:", "title": "Retrograde transport of CDMPR depends on several machineries as analyzed by sulfatable nanobodies.", "author": "Dominik P Buser, Gaétan Bader, Martin Spiess", "journal": "Life Sci Alliance. 2022 Jul; 5(7): e202101269."}, {"pmid": 31331834, "intro": "This antibody (previous batch) has been successfully used in Western blot on Human:", "title": "Quantitative proteomics reveals reduction of endocytic machinery components in gliomas", "author": "Dominik Pascal Buser, Marie-Françoise Ritz, Suzette Moes; Cristobal Tostado, Stephan Frank, Martin Spiess, Luigi Mariani, Paul Jenö, Jean-Louis Boulay, Gregor Hutter", "journal": "https://doi.org/10.1016/j.ebiom.2019.07.039"}]

Goat Anti-AP2A1 (aa706-727) Antibody

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