Goat Anti-SRRT / ARS2 Antibody

$423.00

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SKU: EB12143
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
serrate RNA effector molecule homolog|arsenite-resistance protein 2|arsenite resistance protein|arsenate resistance protein ARS2|arsenate resistance protein 2|serrate|ASR2|ARS2|serrate RNA effector molecule homolog (Arabidopsis)|SRRT
Accession ID
NP_056992.4; NP_001122324.1; NP_001122325.1; NP_001122326.1
Blocking Peptide
EBP12143
Immunogen
Peptide with sequence C-DPRAIVEYRDLDAPD, from the C Terminus of the protein sequence according to NP_056992.4; NP_001122324.1; NP_001122325.1; NP_001122326.1.
Product Comments
This antibody is expected to recognize all reported isoform (NP_056992.4; NP_001122324.1; NP_001122325.1; NP_001122326.1).
Peptide Sequence
C-DPRAIVEYRDLDAPD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human, Mouse, Rat
Human Gene ID
51593
Mouse Gene ID
83701
Rat Gene ID
686980
Product Grade
IHC Results
Paraffin embedded Human Uterus. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 140kDa band observed in lysates of cell line Daudi and of Mouse Spleen, and approx 150kDa band observed in lysates of Rat Testis (calculated MW of 101kDa according to Human NP_056992.4, 100kDa according to Mouse NP_113582.1 and 88.4kDa according to Rat XP_006221478.1). The observed molecular weight is explained by the acetylation of the protein and it corresponds to earlier findings from sources. An additional band of unknown identity was also consistently observed at 37kDa in Mouse Spleen. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC