Goat Anti-Prdm9 (mouse, aa440-452) Antibody

Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.

100 µg

Aliquot and store at -20°C. Minimize freezing and thawing.

meiosis-induced factor containing a PR/SET domain and zinc-finger motif|hybrid sterility protein 1|histone-lysine N-methyltransferase PRDM9|VPR domain containing 9|PR-domain zinc finger protein|PR domain-containing protein 9|PR domain zinc finger protein 9|repro7|Rcr1|Meisetz|G1-419-29|Dsbc1|BC012016|RP23-64O5.3|PR domain containing 9|Prdm9

NP_659058.2

EBP12163

Peptide with sequence C-QEQHVDSQNKNDK, from the internal region of the protein sequence according to NP_659058.2.

C-QEQHVDSQNKNDK

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Refrigerated

Mouse

213389

Antibody detection limit dilution 1:128000.

Preliminary experiments gave an approx 70kDa band in Mouse fetal Brain lysates and in lysates of cell line NIH3T3 after 0.1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.8kDa according to NP_659058.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Pep-ELISA