Goat Anti-ATP5A1 Antibody

$423.00

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SKU: EB12389
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
ATP synthase, H+ transportin|ATP synthase subunit alpha, mitochondrial|ATP synthase alpha chain, mitochondrial|hATP1|ORM|OMR|MOM2|ATPM|ATP5AL2|ATP5A|ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle|ATP5A1
Usage Summary
Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Accession ID
NP_004037.1; NP_001244263.1; NP_001001935.1
Blocking Peptide
EBP12389
Immunogen
Peptide with sequence C-RVHGLRNVQAEE, from the internal region (near N terminus) of the protein sequence according to NP_004037.1; NP_001244263.1; NP_001001935.1.
Product Comments
This antibody is expected to recognize all reported isoforms (NP_004037.1; NP_001244263.1; NP_001001935.1). Reported variants represent identical protein: NP_001244264.1, NP_001001935.1. Reported variants represent identical protein: NP_004037.1, NP_001001937.1 .
Peptide Sequence
C-RVHGLRNVQAEE
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human, Mouse, Rat, Pig
Human Gene ID
498
Mouse Gene ID
11946
Rat Gene ID
65262
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 55kDa band observed in Human, Mouse, Rat and Pig Heart lysates (calculated MW of 54.5kDa according to NP_001001935.1). Recommended concentration: 0.01-0.03µg/ml.
Application Type
Pep-ELISA, WB
Selected References
[{"pmid": 30377371, "intro": "This antibody has been successfully used in the following paper:", "title": "A high-throughput pipeline for validation of antibodies", "author": "Krzysztof Sikorski, Adi Mehta, Marit Inngjerdingen, Flourina Thakor, Simon Kling, Tomas Kalina, Tuula A. Nyman, Maria Ekman Stensland, Wei Zhou, Gustavo A. De Souza, Lars Holden, Jan Stuchly, Markus Templin and Fridtjof Lund-Johansen", "journal": "Nat Methods. 2018 Nov;15(11):909-912"}]