Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names peripherin 1\|Prph1\|peripherin\|Prph
Accession ID NP_038667.2; NP_001157060.1; NP_001157061.1
Blocking Peptide EBP12405
Immunogen Peptide with sequence C-QKEQHSDLDKSSIH, from the C Terminus of the protein sequence according to NP_038667.2; NP_001157060.1; NP_001157061.1.
Product Comments This antibody is expected to recognize all reported isoforms (NP_038667.2; NP_001157060.1; NP_001157061.1).
Peptide Sequence C-QKEQHSDLDKSSIH
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Mouse, Rat
Reactive Species Mouse, Rat
Mouse Gene ID 19132
Rat Gene ID 24688
Product Grade
IHC Results This antibody has been successfully used in IHC on Mouse, PMID: 36226085.
ELISA Detection Limit Antibody detection limit dilution 1:128000.
Western Blot Approx 55kDa band observed in Rat Colon lysates (calculated MW of 54.0kDa according to NP_036765.2). Recommended concentration: 0.1-0.3µg/ml. An additional faint band of unknown identity was also consistently observed at 75kDa. This band was successfully blocked by incubation with the immunizing peptide. Primary incubation was 1 hour.
Application Type Pep-ELISA, WB, IHC

Selected References

[{"pmid": 36226085, "intro": "This antibody has been successfully used in IHC on Mouse:", "title": "Noise-induced hearing loss vulnerability in type III intermediate filament peripherin gene knockout mice.", "author": "Jennie M E Cederholm, Kristina E Parley, Chamini J Perera, Georg von Jonquieres, Jeremy L Pinyon, Jean-Pierre Julien, David K Ryugo, Allen F Ryan, Gary D Housley", "journal": "Front Neurol. 2022 Sep 26;13:962227."}, {"pmid": 0, "intro": "This antibody has been successfully used in the following paper:", "title": "Expression of the P2X1 Receptor Converges onto the Type II Spiral Ganglion Neurons in the Mature Rat Cochlea", "author": "Prakansha N Kumar, Srdjan M Vlajkovic, Peter R Thorne, Haruna Suzuki-Kerr", "journal": "https://doi.org/10.21203/rs.3.rs-6272432/v1 (2025)"}]

Goat Anti-Peripherin 1 (mouse) Antibody

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