Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
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Unit Size 100 µg | |
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing. | |
Synonym / Alias Names polycomb |polycomb group RING finger protein 4|polycomb complex protein BMI-1|murine leukemia viral (bmi-1) oncogene homolog|flvi-2/bmi-1|B lymphoma Mo-MLV insertion region 1 homolog|RNF51|PCGF4|FLVI2/BMI1|BMI1 polycomb ring finger oncogene|BMI1 | |
Accession ID NP_005171.4 | |
Blocking Peptide EBP12495 | |
Immunogen Peptide with sequence C-SHQRDGLTNAGELES, from the internal region of the protein sequence according to NP_005171.4. | |
Peptide Sequence C-SHQRDGLTNAGELES | |
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. | |
Shipping Instructions Refrigerated | |
Predicted Species Human, Mouse, Dog, Pig | |
Reactive Species Human, Mouse, Pig | |
Human Gene ID 648 | |
Mouse Gene ID 12151 | |
Product Grade  | |
IHC Results Paraffin embedded Human Testis. Recommended concentration: 5µg/ml. | |
ELISA Detection Limit Antibody detection limit dilution 1:128000. | |
Western Blot Approx 40kDa band observed in nuclear lysates of cell lines K562 and NIH3T3 (calculated MW of 36.9kDa according to NP_005171.4). This band corresponds to earlier findings in literature with different antibodies (Chagraoui et al, Genes Dev. 2006 Aug 1;20(15):2110-20. PMID: 16882984). Recommended concentration: 0.1-0.3µg/ml.
Approx 37kDa band observed in Pig Bone Marrow lysates (calculated MW of 36.9kDa according to NP_001272900.1 ). Recommended concentration: 1-3µg/ml. | |
Application Type Pep-ELISA, WB, IHC |
Goat Anti-BMI1 (aa237-251) Antibody
$423.00
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