Goat Anti-CD96 Antibody

$255.00

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SKU: EB12532
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
CD96|CD96 molecule|TACTILE|CD96 antigen|T cell activation, increased late expression|T-cell surface protein tactile|cell surface antigen CD96|t cell-activated increased late expression protein
Accession ID
NP_937839.1; NP_005807.1
Blocking Peptide
EBP12532
Immunogen
Peptide with sequence KYTCIQEPNESD, from the C Terminus of the protein sequence according to NP_937839.1; NP_005807.1.
Product Comments
This antibody is expected to recognize both reported isoforms (NP_937839.1; NP_005807.1).
Peptide Sequence
KYTCIQEPNESD
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Human Gene ID
10225
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Preliminary experiments gave an approx 100+75kDa bands in Jurkat lysates after 0.3µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 65.6kDa according to NP_937839.1. The 100kDa and 75kDa bands were successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates?
Application Type
Pep-ELISA