Goat Anti-CTNNB1 / catenin beta-1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB12542
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
catenin beta-1|armadillo|MRD19|CTNNB|catenin (cadherin-associated protein), beta 1, 88kDa|CTNNB1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the cytoplasm and membranes of U251 and A431 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.

Accession ID
NP_001895.1; NP_001317658.1
Blocking Peptide
EBP12542
Immunogen
Peptide with sequence ETARCTAGTLHNLS, from the internal region of the protein sequence according to NP_001895.1; NP_001317658.1.
Product Comments
Reported variants represent identical protein: NP_001091679.1, NP_001895.1, NP_001091680.1.
Peptide Sequence
ETARCTAGTLHNLS
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human
Human Gene ID
1499
Mouse Gene ID
12387
Rat Gene ID
84353
Product Grade
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 90kDa band observed in A431 cell lysates-and in preliminary testing of CACO2 cell lysate (calculated MW of 85.5kDa according to NP_001895.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IF, FC

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