Goat Anti-AIFM1 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB12622
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
striatal apoptosis-inducing factor|programmed cell death 8 (apoptosis-inducing factor)|apoptosis-inducing factor 1, mitochondrial|PDCD8|COXPD6|COWCK|CMTX4|AIF|apoptosis-inducing factor, mitochondrion-associated, 1|AIFM1
Usage Summary
Immunofluorescence: Strong expression of the protein seen in the Mitochondria of HeLa and U2OS cells. Recommended concentration: 10µg/ml.
Accession ID
NP_004199.1; NP_665811.1; NP_665812.1; NP_001124318.1
Blocking Peptide
EBP12622
Immunogen
Peptide with sequence C-NEVAKLFNIHED, from the C Terminus of the protein sequence according to NP_004199.1; NP_665811.1; NP_665812.1; NP_001124318.1.
Product Comments
This antibody is expected to recognize isoform 1 (NP_004199.1), isoform 2 (NP_665811.1), isoform 3 (NP_665812.1) and isoform 4 (NP_001124318.1).
Peptide Sequence
C-NEVAKLFNIHED
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human, Mouse, Rat, Dog, Pig, Cow
Reactive Species
Human, Mouse, Rat, Pig
Human Gene ID
9131
Mouse Gene ID
26926
Rat Gene ID
83533
Product Grade
IHC Results
Paraffin embedded Human Liver. Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:128000.
Western Blot
Approx 70kDa band observed in lysates of cell line Jurkat and NIH3T3, and in Mouse and Rat Heart and Kidney lysates. Approx. 65kDa observed in Pig Heart lysates (calculated MW of 66.9kDa according to Human NP_004199.1 and 66.8kDa according to Mouse NP_036149.1, Rat NP_112646.1 and Pig NP_001284561.1). Recommended concentration: 0.01-0.1µg/ml. Primary incubation 1 hour at room temperature.
Application Type
Pep-ELISA, WB, IHC, IF

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