Goat Anti-HSPA5 / GRP78 Antibody

$464.00

Category: Primary Antibodies
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SKU: EB12857
Formulation
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size
100 µg
Storage Instructions
Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names
immunoglobu|epididymis secretory sperm binding protein Li 89n|endoplasmic reticulum lumenal Ca(2+)-binding protein grp78|78 kDa glucose-regulated protein|MIF2|HEL-S-89n|GRP78|BIP|heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa)|HSPA5
Usage Summary
Immunofluorescence: Expression of the protein seen in the cytoplasm of HeLa cells. Recommended concentration: 5µg/ml
Accession ID
NP_005338.1
Blocking Peptide
EBP12857
Immunogen
Peptide with sequence C-ESHQDADIEDFK, from the internal region (near C Terminus) of the protein sequence according to NP_005338.1.
Peptide Sequence
C-ESHQDADIEDFK
Purification Method
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions
Refrigerated
Predicted Species
Human
Reactive Species
Human, Mouse, Rat
Human Gene ID
3309
Product Grade
IHC Results
Paraffin embedded Human Brain (Cortex). Recommended concentration: 5µg/ml.
ELISA Detection Limit
Antibody detection limit dilution 1:16000.
Western Blot
Approx 75kDa band observed in lysates of cell lines HepG2 and MCF7 and in Mouse Liver lysates, and approx. 80kDa band in Mouse Testis and Rat Brain lysates (calculated MW of 72.3kDa according to Human NP_005338.1, Mouse NP_071705.3 and Rat NP_037215.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation was 1 hour.
Application Type
Pep-ELISA, WB, IHC, IF

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