Formulation Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Unit Size 100 µg
Storage Instructions Aliquot and store at -20°C. Minimize freezing and thawing.
Synonym / Alias Names protein p19\|protein 8.5\|neuron specific gene family member 2\|Nsg2
Usage Summary Immunocytochemistry: This antibody has been successfully used in ICC on Rat: Chan Choo Yap et al. (2022) PMID: 35474277.
Accession ID NP_032767.1
Blocking Peptide EBP12967
Immunogen Peptide with sequence C-HEPKPPKTQGH, from the C Terminus of the protein sequence according to NP_032767.1.
Product Comments Reported variants represent identical protein: NP_001277610.1, NP_001277609.1, NP_032767.1
Peptide Sequence C-HEPKPPKTQGH
Purification Method Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Shipping Instructions Refrigerated
Predicted Species Mouse
Reactive Species Mouse, Rat
Mouse Gene ID 18197
Product Grade
ELISA Detection Limit Antibody detection limit dilution 1:128000.
Western Blot Approx 19kDa band observed in Mouse fetal Brain lysates (calculated MW of 19.0kDa according to NP_032767.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour. This antibody has been successfully used in WB on Rat: Chan Choo Yap et al. (2017) PMID: 28874679.
Application Type Pep-ELISA, WB, ICC

Selected References

[{"pmid": 28874679, "intro": "This antibody has been successfully used in Western blot on Rat:", "title": "The endosomal neuronal proteins Nsg1/NEEP21 and Nsg2/P19 are itinerant, not resident proteins of dendritic endosomes.", "author": "Chan Choo Yap, Laura Digilio, Lloyd McMahon & Bettina Winckler", "journal": "Scientific Reports, 7: 10481, DOI: 10.1038/s41598-017-07667-x (2017)"}, {"pmid": 35474277, "intro": "This antibody has been successfully used in ICC on Rat:", "title": "Dynein is required for Rab7-dependent endosome maturation, retrograde dendritic transport, and degradation", "author": "Chan Choo Yap, Laura Digilio, Lloyd P McMahon, Tuanlao Wang, Bettina Winckler", "journal": "J Neurosci. 2022 Apr 26;JN-RM-2530-21."}]

Goat Anti-Nsg2 (mouse, C terminus) Antibody

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