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Goat Anti-ABCD1 Antibody (EB08708)

Code Name Applications Availability Size Tested species Price Grade  
EB08708 Goat Anti-ABCD1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB08708 1
HEK293 overexpressing human ABCD1 and probed with EB08708 at 1ug/ml (mock transfection in first lane).
Click here to download PDF datasheet.

Target Protein

Principal Names: ABCD1, ATP-binding cassette, sub-family D (ALD), member 1, ABC42, ALD, ALDP, AMN, adrenoleukodystrophy protein
Official Symbol: ABCD1
Accession Number(s): NP_000024.2
Human GeneID(s): 215

Immunogen

Peptide with sequence C-EDMQRKGYSEQD, from the internal region of the protein sequence according to NP_000024.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Preliminary experiments gave an approx. 50kDa band in Human Brain (Cerebellum) lysates after 1µg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 82.9kDa according to NP_000024.2. The 50kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?

Species Reactivity

Tested: Human
Expected from sequence similarity: Human