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Code Name Applications Availability Size Tested species Price Grade  
EB05674 Goat Anti-ACADM Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05674 1
EB05674 staining (0.05µg/ml) of Human Heart lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
EB05674 2
EB05674 (3.75µg/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
EB05674 3
EB05674 (3.75µg/ml) staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: ACADM, acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain, ACAD1, MCAD, MCADH, medium-chain acyl-CoA dehydrogenase, FLJ18227, FLJ93013, FLJ99884, medium-chain specific acyl-CoA dehydrogenase, acyl-Coenzyme A dehydrogenase, C-4 to C-12 straight chain
Official Symbol: ACADM
Accession Number(s): NP_000007.1; NP_001120800.1
Human GeneID(s): 34
Important Comments: This antibody is expected to recognise both reported isoforms (NP_000007.1; NP_001120800.1).

Immunogen

Peptide with sequence C-RLIVAREHIDKYKN, from the C Terminus of the protein sequence according to NP_000007.1; NP_001120800.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 45kDa band observed in Human Kidney and Heart lysates (calculated MW of 46.6kDa according to NP_000007.1). Recommended for use at 0.01-0.1µg/ml. Primary incubation was for 1 hour.
IHC: Paraffin embedded Human Liver and Adrenal Gland. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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