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Code Name Applications Availability Size Tested species Price Grade  
EB13087 Goat Anti-ACE2 (N Terminal) Antibody Pep-ELISA, WB, IHC, FC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: [email protected]
www.arp1.com
EB13087 1
EB13087 (1.5µg/ml) staining of HepG2 cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB13087 2
EB13087 (0.01µg/ml) staining of recombinant hACE2 (1 µg protein in PBS, pH 7.4). Detected by chemiluminescence.
EB13087 3
EB13087 (8µg/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB13087 4
EB13087 Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.
EB13087 5
EB13087 Flow cytometric analysis of paraformaldehyde fixed HepG2 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: ACE 2 antibody, ACE related carboxypeptidase antibody, ACE-related carboxypeptidase antibody, ACE2 antibody, ACE2_HUMAN antibody, ACEH antibody, Angiotensin converting enzyme 2 antibody, Angiotensin converting enzyme homolog antibody, Angiotensin converting enzyme like protein antibody, Angiotensin I Converting Enzyme (peptidyl dipeptidase A)
Official Symbol: ACE2
Accession Number(s): NP_001358344.1
Human GeneID(s): 59272

Immunogen

Peptide with sequence PROPRIETARY,-C, from the N Terminus of the protein sequence according to NP_001358344.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 90-100kDa band observed in lysates of cell line HepG2 (calculated MW of 92.4kDa according to NP_001358344.1). Recommended concentration 0.5-2ug/ml. Primary incubation 1 hour at room temperature.

Approx 100kDa band was also observed on full-length recombinant Human ACE2 protein. Recommended concentration 0.01-0.1ug/ml. Primary incubation 1 hour at room temperature.


IHC: Paraffin embedded Human Testis. Recommended concentration: 8µg/ml.

Flow Cytometry: Flow cytometric analysis of HepG2 cells.. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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