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Code Name Applications Availability Size Tested species Price Grade  
EB09857 Goat Anti-Aconitase 2 Antibody Pep-ELISA, WB, IF, IHC Yes 100µg specific antibody in 200µl Human $450.00 Elite_Plus

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Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB09857 1
EB09857 (0.01µg/ml) staining of Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB09857 2
EB09857 (3.8µg/ml) staining of paraffin embedded Human Breast. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: aconitase 2, mitochondrial, ACONM, MGC20605, MGC33908, ACO2, aconitase 2, citrate hydro-lyase, epididymis secretory sperm binding protein Li 284, HEL-S-284, ICRD, mitochondrial aconitase, OCA8
Official Symbol: ACO2
Accession Number(s): NP_001089.1
Human GeneID(s): 50
Non-Human GeneID(s): 11429 (mouse), 79250 (rat)

Immunogen

Peptide with sequence C-QHVDVSPTSQRLQ, from the internal region of the protein sequence according to NP_001089.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 90kDa band observed in Human Heart lysates (calculated MW of 85.4kDa according to NP_001089.1). Recommended concentration: 0.01-0.03µg/ml.
IHC: In paraffin embedded Human Breast shows mitochondrial staining in the epithelial cells of the ducts. Recommended concentration, 3-5µg/ml.

Immunofluorescence: Customer finds weak pixulate staining in the cytoplasm of HeLa cells.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig

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