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Code Name Applications Availability Size Tested species Price Grade  
EB11059 Goat Anti-ADAM12 (aa225-239) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse, Rat, Pig $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB11059 1
EB11059 (0.1µg/ml) staining of Human Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB11059 2
EB11059 (0.1µg/ml) staining of Mouse (A), Rat (B) and Pig (C) Heart lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: ADAM metallopeptidase domain 12, disintegrin and metalloproteinase domain-containing protein 12, MCMP, MCMPMltna, meltrin-alpha, MLTN, MLTNA, ADAM12
Official Symbol: ADAM12
Accession Number(s): NP_003465.3; NP_067673.2
Human GeneID(s): 8038
Non-Human GeneID(s): 11489 (mouse), 679837 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_003465.3; NP_067673.2).

Immunogen

Peptide with sequence C-REFQRQGKDLEKVKQ, from the internal region of the protein sequence according to NP_003465.3; NP_067673.2.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 100kDa band observed in Human Heart lysates and approx 90+60kDa bands observed in Mouse, Rat and Pig Heart lysates (calculated MW of 99.5kDa according to NP_003465.3 and of 80.4kDa according to NP_067673.2). Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Mouse, Rat, Pig
Expected from sequence similarity: Human, Mouse, Rat, Pig, Cow

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