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Goat Anti-ADAM17 / TACE Antibody (EB05382)

Code Name Applications Availability Size Tested species Price Grade  
EB05382 Goat Anti-ADAM17 / TACE Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $333.00 Elite

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ARP American Research Products, Inc

ARP American Research Products, Inc
135 Beaver Street, Suite 204
Waltham, MA 02452
Ph. (800) 832-2611
Fax. (888) 832-2613
Email: info@arp1.com
www.arp1.com

EB05382 1
EB05382 staining (0.2µg/ml) of Human HeLa lysate (RIPA buffer, 35µg total protein per lane). Detected by chemiluminescence.
EB05382 2
EB05382 (3.75µg/ml) staining of paraffin embedded Human Skin. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
EB05382 3
EB05382 (3.75µg/ml) staining of paraffin embedded Human Thyroid. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch, not on sale.
Click here to download PDF datasheet.

Target Protein

Principal Names: ADAM17, TACE, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), CSVP, cSVP, CD156b, CD156b, MGC71942, TACE, cSVPADAM metallopeptidase domain 17, TNF-alpha converting enzyme, a disintegrin and metalloproteinase domain 17, a disintegrin and metalloproteinase domain 17 (tumor necrosis factor, alpha, converting enzyme), snake venom-like protease, ADAM metallopeptidase domain 17, ADAM18, CD156B, ADAM metallopeptidase domain 18, snake venom-like protease, tumor necrosis factor, alpha, converting enzyme
Official Symbol: ADAM17
Accession Number(s): NP_003174.3
Human GeneID(s): 6868
Non-Human GeneID(s): 11491 (mouse)
Important Comments: This antibody is expected to recognise isoform 1 (represented by NP_003174.3) and not isoform 2 (represented by NP_068604).

Immunogen

Peptide with sequence LQRQNRVDSKETEC, from the C Terminus of the protein sequence according to NP_003174.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Approx 125-130kDa band observed in Hela, Jurkat and U937 lysates (predicted MW of kDa according to NP_003174.3 but glycosylated). Recommended for use at 0.1-1µg/ml. Primary incubation was for 1 hour. The observed molecular weights correspond to earlier findings in literature with different antibodies (Le Gall et al, J Cell Sci. 2010 Nov 15;123(Pt22):3913-22; PMID: 20980382)

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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