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Goat Anti-AES Antibody (EB10957)

Related Products: EBP10957 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB10957 Goat Anti-AES Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB10957 1
EB10957 (0.3µg/ml) staining of Human Cerebral Cortex lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10957 2
EB10957 (3.75µg/ml) staining of paraffin embedded Human Colon. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: AES, amino-terminal enhancer of split, AES-1, AES-2, ESP1, GRG, GRG5, TLE5, amino enhancer of split, gp130-associated protein GAM
Official Symbol: AES
Accession Number(s): NP_945320.1; NP_001121.2; NP_945321.1
Human GeneID(s): 166
Non-Human GeneID(s): 14797 (mouse), 29466 (rat)
Important Comments: This antibody is expected to recognize all reported isoforms (NP_945320.1; NP_001121.2; NP_945321.1).

Immunogen

Peptide with sequence SHLPQQLKFTTSD-C, from the N Terminus of the protein sequence according to NP_945320.1; NP_001121.2; NP_945321.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 30kDa band observed in Human Brain (Cerebellum and Cerebral Cortex) lysates (calculated MW of 29.1kDa according to NP_945320.1). Recommended concentration: 0.3-1µg/ml.
IHC: Paraffin embedded Human Colon. Recommended concentration: 3.75µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat

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