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Goat Anti-AGR2 Antibody (EB07494)

Code Name Applications Availability Size Tested species Price Grade  
EB07494 Goat Anti-AGR2 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $423.00 Elite

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EB07494 1
EB07494 (0.1µg/ml) staining of Human Colon lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
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Target Protein

Principal Names: AGR2, anterior gradient 2 homolog (Xenopus laevis), AG2, GOB-4, HAG-2, XAG-2, anterior gradient 2 homolog, anterior gradient 2 homolog (Xenepus laevis), secreted cement gland homolog
Official Symbol: AGR2
Accession Number(s): NP_006399.1
Human GeneID(s): 10551
Non-Human GeneID(s): 23795 (mouse)

Immunogen

Peptide with sequence C-GAKKDTKDSRPK, from the internal region of the protein sequence according to NP_006399.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx. 18kDa band observed in Human Colon, Duodenum and Lung lysates (calculated MW of 20.0kDa according to NP_006399.1). Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog