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Code Name Applications Availability Size Tested species Price Grade  
EB05419-B Goat Anti-AIF1 / IBA1 (isoform 1 and 3), biotinylated Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05419-B 1
Biotinylated EB05419 (0.1µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.
Click here to download PDF datasheet.

Target Protein

Principal Names: AIF1, allograft inflammatory factor 1, AIF-1, IBA1, IRT-1, IRT1, allograft inflammatory factor-1 splice variant Hara-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, protein G1, DADB-70P7.8, Daintain
Official Symbol: AIF1
Accession Number(s): NP_116573.1; NP_004838.1; NP_001614.3
Human GeneID(s): 199
Non-Human GeneID(s): 11629 (mouse), 29427 (rat)
Important Comments: This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Immunogen

Peptide with sequence C-TGPPAKKAISELP., from the C Terminus of the protein sequence according to NP_116573.1; NP_004838.1; NP_001614.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
Western blot: Approx 17kDa band observed in Rat Brain lysates (calculated MW of 16.7kDa according to NP_001614.3). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 0.1-0.3µg/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Pig

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