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Code Name Applications Availability Size Tested species Price Grade  
EB05419-H Goat Anti-AIF1/IBA1 isoform 1 and 3 (Human reactivity only) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $300.00 Elite

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EB05419-H 1
EB05419-H (2ug/ml) staining of Human Frontal Cortex lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB05419-H 2
EB05419-H (4µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
EB05419-H 3
EB05419-H Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
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Target Protein

Principal Names: AIF1, IBA1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, Daintain, DADB-70P7.8, allograft inflammatory factor-1 splice variant Hara-1, IRT1, protein G1
Official Symbol: AIF1
Accession Number(s): NP_116573.1; NP_001614.3
Human GeneID(s): 199
Important Comments: This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Immunogen

Peptide with sequence C-TGPPAKKAISELP.., from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 18kDa band observed in Human Frontal Cortex lysates (calculated MW of 16.7kDa according to NP_001614.3). Recommended concentration: 1-3µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Spleen. Recommended concentration: 4µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human