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Goat Anti-AIF1/IBA1 isoform 1 and 3 (Rat reactivity only) Antibody (EB05419-R)

Related Products: EBP05419-R - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB05419-R Goat Anti-AIF1/IBA1 isoform 1 and 3 (Rat reactivity only) Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Rat $300.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB05419-R 1
EB05419 (2.0µg/ml) staining of Rat Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: AIF1, IBA1, allograft inflammatory factor 1, AIF-1, IRT-1, interferon gamma responsive transcript, ionized calcium-binding adapter molecule 1, Daintain, DADB-70P7.8, allograft inflammatory factor-1 splice variant Hara-1, IRT1, protein G1
Official Symbol: AIF1
Accession Number(s): NP_116573.1; NP_001614.3
Non-Human GeneID(s): 29427 (rat)
Important Comments: This antibody is expected to recognize isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Immunogen

Peptide with sequence C-TGPPAKKAISELP.,, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx 16kDa band observed in Rat Brain lysates (calculated MW of 16.8kDa according to Rat NP_058892.1). Recommended concentration:1-3ug/ml. Primary incubation 1 hour at room temperature.

Species Reactivity

Tested: Rat
Expected from sequence similarity: Rat

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