Choose your country here (United States of America)
Home
Code Name Applications Availability Size Tested species Price Grade  
EB07174 Goat Anti-AIF1 / IBA1 (isoform 3) Antibody Pep-ELISA, IF, FC, IHC Yes 100µg specific antibody in 200µl Human $464.00 Elite_Plus

Ordering Information for United States of America (change)

Order direct from Everest

$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

Order offline
EB07174 1
EB07174 Immunofluorescence analysis of paraformaldehyde fixed U937 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07174 2
EB07174 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
EB07174 3
EB07174 Flow cytometric analysis of paraformaldehyde fixed U937 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB07174 4
EB07174 (7µg/ml) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
EB07174 5
EB07174 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.
EB07174 6
EB07174 (5µg/ml) staining of paraffin embedded Human Cortex. Heat induced antigen retrieval with citrate buffer Ph 6, HRP-staining.
EB07174 7
EB07174 Negative Control showing staining of paraffin embedded Human Cortex, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: AIF1, IBA1, allograft inflammatory factor 1, DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule
Official Symbol: AIF1
Accession Number(s): NP_001614.3
Human GeneID(s): 199
Non-Human GeneID(s): 11629 (mouse)
Important Comments: This antibody is expected to recognise isoform 3 (NP_001614.3) only.

Immunogen

Peptide with sequence C-NKQFLDDPKYSSDED, from the internal region of the protein sequence according to NP_001614.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:128000.
IHC: Paraffin embedded Human Spleen and Brain (Cortex). Recommended concentration: 5-7µg/ml.

Immunofluorescence: Strong expression of the protein seen in the nucleus and cytoplasm of CaCo-2 and U937 cells. Recommended concentration: 10µg/ml.

Flow Cytometry: Flow cytometric analysis of U937 cells. Recommended concentration: 10ug/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Dog

Specific Reference

This antibody (previous batch) has been successfully used in IHC on Rat:
Joshua P. Thaler, Chun-Xia Yi, Ellen A. Schur, Stephan J. Guyenet, Bang H. Hwang, Marcelo O. Dietrich, Xiaolin Zhao, David A. Sarruf, Vitaly Izgur, Kenneth R. Maravilla, Hong T. Nguyen, Jonathan D. Fischer, Miles E. Matsen, Brent E. Wisse, Gregory J. Morton, Tamas L. Horvath, Denis G. Baskin, Matthias H. Tschöp, and Michael W. Schwartz
Obesity is associated with hypothalamic injury in rodents and humans.
J Clin Invest. 2012 Jan 3; 122(1): 153-162.
PMID: 22201683