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Code Name Applications Availability Size Tested species Price Grade  
EB07257 Goat Anti-AIF1 / IBA1 (isoforms 1 + 3) Antibody Pep-ELISA, FC, IF, IHC Yes 100µg specific antibody in 200µl Human $464.00 Elite_Plus

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EB07257 1
EB07257 (4µg/ml) staining of paraffin embedded Human Cerebellum. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.
EB07257 2
EB07257 Flow cytometric analysis of paraformaldehyde fixed K562 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.
EB07257 3
EB07257 Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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Target Protein

Principal Names: AIF1, IBA1, allograft inflammatory factor 1, DASS-82G15.2, AIF-1, IRT-1, Em:AF129756.17, interferon gamma responsive transcript, ionized calcium-binding adapter molecule
Official Symbol: AIF1
Accession Number(s): NP_116573.1; NP_001614.3
Human GeneID(s): 199
Important Comments: This antibody is expected to recognise isoform 1 (NP_116573.1) and isoform 3 (NP_001614.3).

Immunogen

Peptide with sequence C-YEEKAREKEKP, from the C Terminus of the protein sequence according to NP_116573.1; NP_001614.3.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
IHC: In paraffin embedded Human Cerebellum shows staining of astrocytes. Recommended concentration, 2-4µg/ml.

Flow Cytometry: Flow cytometric analysis of K562 cells. Recommended concentration: 10ug/ml.

Immunofluorescence: Strong expression of the protein seen in the cytoplasm of CaCo-2 cells. Recommended concentration: 10µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human