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Goat Anti-AKAP8 / AKAP95 Antibody (EB06326)

Related Products: EBP06326 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06326 Goat Anti-AKAP8 / AKAP95 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06326 1
EB06326 staining (1µg/ml) of Human Liver lysate (RIPA buffer, 35µg total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Click here to download PDF datasheet.

Target Protein

Principal Names: AKAP8, AKAP95, DKFZp586B1222, A kinase (PRKA) anchor protein 8, A kinase anchor protein 95, A-kinase anchor protein 8, A-kinase anchor protein, 95kDa
Official Symbol: AKAP8
Accession Number(s): NP_005849.1
Human GeneID(s): 10270
Non-Human GeneID(s): 56399 (mouse), 116633 (rat)

Immunogen

Peptide with sequence DQGYGGYGAWSAG-C, from the N Terminus of the protein sequence according to NP_005849.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:32000.
Western blot: Experiments gave a band at approx 100kDa in Human Liver lysate after 1µg/ml antibody staining. This band corresponds to the 95kDa band reported in literature (PMID: 10601332). This protein has a calculated MW of 76.1kDa according to NP_005849. Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human, Mouse, Rat, Cow

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