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Goat Anti-AKT2 Antibody (EB06772)

Related Products: EBP06772 - Immunizing Peptide
Code Name Applications Availability Size Tested species Price Grade  
EB06772 Goat Anti-AKT2 Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human, Rat $411.00 Elite

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Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB06772 1
EB06772 (1µg/ml) staining of Human Skeletal Muscle (A) and (0.75ug/ml) Human Testes (B) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06772 2
EB06772 (1µg/ml) staining of Rat Testes lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB06772 3
EB06772 (4µg/ml) staining of paraffin embedded Human Testis. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.
EB06772 4
EB06772 Negative Control showing staining of paraffin embedded Human Testis, with no primary antibody.
Click here to download PDF datasheet.

Target Protein

Principal Names: AKT2, v-akt murine thymoma viral oncogene homolog 2, HGNC:392, PKBBETA, PRKBB, RAC-BETA, Murine thymoma viral (v-akt) homolog-2, rac protein kinase beta
Official Symbol: AKT2
Accession Number(s): NP_001617.1; NP_001229956.1; NP_001317440.1
Human GeneID(s): 208
Important Comments: This antibody is expectesd to recognise all reported iasoforms NP_001617.1; NP_001229956.1; NP_001317440.1.

Immunogen

Peptide with sequence C-RYDSLGLLELDQRT, from the C Terminus of the protein sequence according to NP_001617.1; NP_001229956.1; NP_001317440.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:16000.
Western blot: Approx. 58-60kDa band observed in Human Skeletal Muscle lysates and in Rat Testis lysates, and approx.60kDa in Human Testis lysates, and in preliminary testing of THP-1 cell lysate (calculated MW of 55.8kDa according to Human NP_001617.1 and 55.5kDa according to Rat NP_058789.1). Recommended concentration: 0.5-1µg/ml. Primary incubation 1 hour at room temperature
IHC: Paraffin embedded Human Testis. Recommended concentration: 4-6µg/ml.

Flow Cytometry: A customer reported weak staining on THP-1 cells at a concentration of 10ug/ml.

Species Reactivity

Tested: Human, Rat
Expected from sequence similarity: Human, Mouse, Rat, Dog

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