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Goat Anti-ALDH18A1 Antibody (EB10433)

Code Name Applications Availability Size Tested species Price Grade  
EB10433 Goat Anti-ALDH18A1 Antibody Pep-ELISA, WB Yes 100µg specific antibody in 200µl Human, Mouse $423.00 Elite

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EB10433 1
EB10433 (1µg/ml) staining of MCF7 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
EB10433 2
EB10433 (0.3µg/ml) staining of NIH3T3 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Target Protein

Principal Names: aldehyde dehydrogenase 18 family, member A1, aldehyde dehydrogenase 18A1, delta1-pyrroline-5-carboxlate synthetase, GSAS, MGC117316, P5CS, PYCS, pyrroline-5-carboxylate synthetase (glutamate gamma-semialdehyde synthetase), ALDH18A1
Official Symbol: ALDH18A1
Accession Number(s): NP_002851.2; NP_001017423.1
Human GeneID(s): 5832
Non-Human GeneID(s): 56454 (mouse), 361755 (rat)
Important Comments: This antibody is expected to recognize both reported isoforms (NP_002851.2; NP_001017423.1).

Immunogen

Peptide with sequence C-SEHGSLKYLH, from the C Terminus of the protein sequence according to NP_002851.2; NP_001017423.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:8000.
Western blot: Approx 85kDa band observed in lysates of cell lines NIH3T3 and MCF7 (calculated MW of 87.3kDa according to NP_002851.2). Recommended concentration: 1-3µg/ml.

Species Reactivity

Tested: Human, Mouse
Expected from sequence similarity: Human, Mouse, Rat, Dog, Cow, Pig