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Goat Anti-AMACR (aa312-326) Antibody (EB12539)

Related Products: EBP12538 - Immunizing Peptide, EBP12539 - Immunizing Peptide, Goat Anti-AMACR Antibody
Code Name Applications Availability Size Tested species Price Grade  
EB12539 Goat Anti-AMACR (aa312-326) Antibody Pep-ELISA, WB, IHC Yes 100µg specific antibody in 200µl Human $411.00 Elite

Ordering Information for

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$73.00: Overnight shipping to United States of America
Sent via overnight courier arrives approximately next day to mainland USA. Please note that your order will be shipped by our parent company Vector Laboratories.

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99% of products are held in stock for immediate shipment, see individual product pages for availability.

Please order through the US Dollar shopping cart (links on each product page) or we accept orders by email ([email protected]) Please include the following information with your order:

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In stock orders received by 11 am PST on Monday through Friday are shipped the same day.

Orders are shipped by our parent company Vector Laboratories, 6737 Mowry Avenue, Newark CA 94560, USA. 
EB12539 1
EB12539 (1µg/ml) staining of CaCo-2 (A) and (0.1ug/ml) LNCaP (B) cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12539 2
EB12538 (0.03µg/ml) staining of Human Kidney (A),,(0.1ug/ml) Liver (B) and (1ug/ml) Colorectal cancer (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.
EB12539 3
EB12539 (5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
Click here to download PDF datasheet.

Target Protein

Principal Names: AMACR, alpha-methylacyl-CoA racemase, AMACRD, CBAS4, RACE, RM, 2-methylacyl-CoA racemase
Official Symbol: AMACR
Accession Number(s): NP_055139.4; NP_001161067.1
Human GeneID(s): 23600

Immunogen

Peptide with sequence C-RGSFITSEEQDVSPR, from the internal region of the protein sequence according to NP_055139.4; NP_001161067.1.

Please note the peptide is available for sale.

Purification and Storage

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Aliquot and store at -20°C. Minimize freezing and thawing.

Applications Tested

Peptide ELISA: antibody detection limit dilution 1:64000.
Western blot: Approx 40-42kDa band observed in Human Kidney, Liver and Coloretal cancer lysates, and in lysates of cell line CaCo-2, and approx 40.45kDa band observed in lysates of cell line LNCaP (calculated MW of 42.4kDa according to NP_055139.4) and 43.9kDa according to .NP_001161067.1) Recommended concentration: 0.03-1µg/ml. Primary incubation 1 hour at room temperature.
IHC: Paraffin embedded Human Kidney. Recommended concentration: 5µg/ml.

Species Reactivity

Tested: Human
Expected from sequence similarity: Human

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